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Part:BBa_K4204003:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-04)


Transcriptional unit of sgRNA targeting HBLA gene of B.cereus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 26
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The last 20 bp is the target region and could be replaced with other sequences that pairs with the target gene.


Source

Designed by BNDS_China 2022, based on [1]'s paper.

References

[1] Li, L., Li, S., Wu, N., Wu, J., Wang, G., Zhao, G., & Wang, J. (2019). HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation. American Chemical Society Synthetic Biology, 8(10), 2228–2237. https://doi.org/10.1021/acssynbio.9b00209